수종의 cytokine이 배양중인 조골세포에 미치는 영향
Effects of Cytokines on the Osteoblastic Cells in Culture
단국대치의학연구소논문집 1995년 7권 1호 p.215 ~ 228
李鐘烈, 高鮮一, 金正根,
소속 상세정보
李鐘烈 ( ) - 檀國大學校 齒醫學硏究所
高鮮一 ( ) - 檀國大學校 齒醫學硏究所
金正根 ( ) - 檀國大學校 齒醫學硏究所
KMID : 0354619950070010215
Abstract
Evidence indicates that cytokines are involved in the inioation and progression of chronic infalmmatory diseasea. Retent evidence demonstrates major roles of 9rowth factors and cytokines as mendiators for bone growth and remodeling. Bone remodeling is a local phenomenon which occurs In discrete packets thoughout the skeleio7. The cellular events which comprise the remodeling seauence are controlled by cytotnnes which are generated In the microenvironment of the bone resorbing area. These cytokines are derived from marrow mononuclear cells or from bene cells themsetves, or they are incorporated into the bone maax and released In biologically active form as bone resorb. But evidence is aofumulating that some of these cytokines play an Imponant rote not just In physiological bone remodeling, but also in commui diseases of bone remodeling such as osteoporosls, osteopetrosis Paget´s diseases and malignant diseases which involve bone and chronic intlammatory diseases such as IteurnaTno arthritis and periodontal
diseases. Also, these cytokines are derived from bone cells themselves, these rryepㄴor,s are nresent in bone cells. Therefore, this expefrment developed a culture of osteoblastic cells from emeryoruc crucken calvaria to examine the possible role of some cytosne iln osteoblast function. This expenment was penormea 1) to examine the effect of cytokines on the acid phosphatase actifty of chicken osteoblast and 2) to examine the ettect of cvtokines on the alkaline phosphatase activity of chicken osteoblast and 3) to study the ettect or cyfolnnes on the bone nodule formation of chicken osteoblast in long-term cultures. The observed results were as follows.
1. Cytokines and cytokine combination stimulate the felease of acid phosphatase into cultured midium in cultures of chicken osteoblastl
2. Cytokines and cytokine combination increase the activity of acid phosphatase of cell extract in cultures of chicken osteoblast.
3. Cytokines and cytokine combination decrease the activity of alkaline phosphatase of cell extract in cultures of chicken osteoblast.
4. Less bone nodules were formed by cytokines than in control group.
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